详细信息:We have developed a multi-parameter flow cytometry assay for ZAP-70 protein expression analysis and characterization of human lymphocytes. Millipore’s FlowCellect™ Human Lymphocyte ZAP-70 Characterization Kit includes three directly conjugated antibodies: CD5 FITC, CD19 APC, and ZAP-70 PE, along with optimized fixation, permeabilization, wash, and assay buffers to provide researchers the ability to phenotypically distinguish cell types.
Antibodies against CD5 and CD19 are provided in the kit to phenotype cells by their surface marker expression. CD5 is normally a pan-T-cell marker, while CD19 is a mature B-cell marker. Some B-cell malignancies express both of these molecules on their surface, and therefore can be distinguished from the two aforementioned cell types.
ZAP-70 is a molecule involved in T-cell activation, which can also be expressed within B-cells at levels correlating to disease severity. The combinatorial staining of cells for all three of these markers assists the researcher studying the molecular biology of T-cell activation or B-cell disease states.
Sufficient reagents are provided for 25 3-color samples. The kit includes all optimized fluorescent labeled antibodies and buffers necessary for cell preparation and analysis.
