详细信息:Millipore's In Vitro Osteogenesis Assay Kit contains all reagents necessary to efficiently differentiate MC3T3-E1cells to a mature osteoblastic lineage, as determined by staining for mineralization. In addition this kit provides all the necessary reagents and a protocol for quantifying osteogenesis using a standard plate reader. This product is useful for studying the effects of growth factors, drugs and toxic agents on bone formation and for screening candidate osteogenic pharmaceuticals. Also, studies examining the intracellular signaling pathways regulating osteoblast differentiation can be facilitated by use of this kit.
The kit includes the osteogenesis-inducing reagents: ascorbic acid 2-phosphate, β-glycerophosphate and melatonin, and Alizarin Red Solution, a staining solution used to detect the presence of calcium in bone. Additionally, we provide reagents and a protocol for performing quantitative analysis of Alizarin Red staining.
Using Millipore's In Vitro Osteogenesis Assay Kit, we routinely obtain >75% mature osteoblasts from a starting population of uninduced MC3T3-E1 cells obtained from ATCC. Efficiency of osteoblastic differentiation may vary, depending upon the quality of the cells used, and if variations to the protocol are introduced.
