详细信息:The OxyIHC™ Oxidative Stress Detection Kit (S7450) contains the chemical and immunological reagents
necessary to detect protein oxidation in various tissues from a variety of organs and animal species. The
OxyIHC test method involves chemical derivatization of protein carbonyl groups with 2,4-
dinitrophenylhydrazine (DNPH). This chemical reaction results in proteins being covalently coupled to DNP
at their carbonyl sites. The DNP-derivatized proteins are then detected using an antibody that specifically
binds to the DNP moiety. Subsequent incubation with biotin conjugated secondary antibody, streptavidin
conjugated HRP, and development using a 3,3’ diaminobenzidine (DAB) staining allows
immunohistochemical detection of protein oxidation.
