详细信息:Recombinant HEK293 cell line expressing the human GABAA α6/β3/γ2 chloride ion channel. HEK293 cells expressing hGABAA α6/β3/γ2 channels were characterized in terms of their pharmacological properties using whole-cell PatchXpress™ and IonWorks™ automated patch clamp platforms.
The cell line was shown to be selectively expressing hGABAA α6/β3/γ2 currents since large inward currents were seen upon exposure to GABA in the extracellular solution in cells held at -40 mV. These currents were blocked by bicuculline, a specific GABAA channel antagonist. In addition, an EC20 application of GABA elicited currents that were increased by the positive allosteric modulator RO 15-4513.
Functional channel expression over time was monitored using IonWorks™ HT. Channel expression is robust over at least 26 passages: 93.37% of cells expressed hGABAA α6/β3/γ2 currents at passage 30 giving a mean current amplitude at –40 mV of 1.54 ± 0.9 nA (n=169).
