详细信息:Recombinant HEK293 cell line expressing the human GABAA α4/β3/γ2 chloride ion channel. HEK293 cells expressing hGABAA α4/β3/γ2 channels were characterized in terms of their pharmacological properties using whole-cell PatchXpress™ and IonWorks™ automated patch clamp platforms.
The cell line was shown to be selectively expressing hGABAA α4/β3/γ2 currents since large inward currents were seen upon exposure to GABA in the extracellular solution in cells held at -40 mV. These currents were blocked by bicuculline, a specific GABAA channel antagonist. In addition, an EC20 application of GABA elicited currents that were positively modulated by RO 15-4513, but not by diazepam.
Functional channel expression over time was monitored using IonWorks™ HT. Channel expression is robust over at least 28 passages: 88 % of cells expressed hGABAA α4/β3/γ2 currents at passage 28 giving a mean current amplitude at –40 mV of 1.28 ± 1.6 nA (n=330).
