应用:Description
The Phospho-Estrogen Receptor α Antibody Sampler Kit provides an economical means to evaluate the activation status of ERα, including phosphorylation of Ser104/106, Ser167, and Ser118. The monoclonal control ERα antibody is also included to detect total Estrogen Receptor α levels. The kit contains enough primary antibody to perform four Western blot experiments.Phospho-Estrogen Receptor α Antibody Sampler Kit提供一种经济的方法来评估雌激素受体α的激活状态，包括丝氨酸（104/106，167和118位）的磷酸化。monoclonal control ERα antibody还包括能够检测总的雌激素受体α水平。该试剂盒包含足够的一抗，可以完成4次Western blot实验。
详细信息:Specificity / Sensitivity
Phospho-Estrogen Receptor α (Ser167) (D1A3) Rabbit mAb detects endogeneous levels of ERα protein only when phosphorylated at Ser167, and also cross reacts with a nonspecific band around 77 kDa. Phospho-Estrogen Receptor α (Ser104/106) Antibody detects endogenous levels of Ser104/106 phosphorylated ERα. Phospho-Estrogen Receptor α (Ser118) (16J4) Mouse mAb will detect endogenous levels of Ser118 phosphorylated ERα. Estrogen Receptor α (D8H8) Rabbit mAb detects endogenous levels of ERα. Each antibody in the kit does not cross-react with phosphorylated or nonphosphorylated ER isoforms β or other related family members.只有当丝氨酸（167位）磷酸化时，Phospho-Estrogen Receptor α (Ser167) (D1A3) Rabbit mAb能够检测内源性水平的雌激素受体α蛋白，并与约77 kDa的非特异性条带交叉反应。Phospho-Estrogen Receptor α (Ser104/106) Antibody能够检测内源性水平的丝氨酸（104/106位）磷酸化的雌激素受体α。Phospho-Estrogen Receptor α (Ser118) (16J4) Mouse mAb将能够检测内源性水平的丝氨酸（118位）磷酸化的雌激素受体α。Estrogen Receptor α (D8H8) Rabbit mAb能够检测内源性水平的雌激素受体α。试剂盒中的每个抗体都不与磷酸化或非磷酸化的雌激素受体β亚型或其他相关家族成员发生交叉反应。
Source / Purification
Activation state monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides or non-phosphopeptide corresponding to residues surrounding Ser167, Ser118, and the carboxy terminus of human estrogen receptor α protein. Activation state polyclonal antibody is produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser104/106 of human ERα. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.该激活状态的单克隆抗体通过用合成磷酸肽或非磷酸肽免疫动物制备，该合成磷酸肽是人雌激素受体α蛋白丝氨酸（167，118位）附近的残基和靠近羧基末端的残基。该激活状态的多克隆抗体通过用合成磷酸肽免疫动物制备，该合成磷酸肽是人雌激素受体α蛋白丝氨酸（104/106位）附近的残基。多克隆抗体由蛋白A和肽亲和层析纯化。
