详细信息:The EasySep™ Mouse Epithelial Cell Enrichment Kit is designed to isolate epithelial cells from freshly dissociated mouse mammary tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies that are directed against non-epithelial cells (CD45, CD31, TER119, BP-1). Labeled cells are then recognized by Tetrameric Antibody Complexes that are directed against biotin and dextran. These cells are bound to magnetic particles and separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. Components: ™ EasySep™ Mouse Epithelial Cell Enrichment Cocktail, 0.5 mL
™ EasySep™ Biotin Selection Cocktail, 1.0 mL
™ EasySep™ Magnetic Particles, 1.0 mL
Equipment Required: ™ EasySep™ Magnet (Catalog #18000), or
™ “The Big Easy” EasySep™ Magnet (Catalog #18001)
