详细信息:The EasySep™ Human Th17 Enrichment Kit is a two-step method designed to enrich human Th17 cells from fresh peripheral blood nucleated cells. CD4+CXCR3- T cells are first pre-enriched by negative selection using EasySep™ Human CD4+CXCR3- T Cell Pre-Enrichment Cocktail. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes (TAC) recognizing CD8, CD14, CD16, CD19, CD20, CD36, CD56, CD66b, CD123, TCR™/d, glycophorin A, CD45RAhigh, CXCR3 and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. Following pre-enrichment, CCR6 positive CD4+CXCR3- T cells are selected using EasySep™ Human CCR6 Positive Selection Cocktail. Cells are targeted with TAC recognizing CCR6 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet and remain in the tube while the unwanted cells are poured off. The selection steps can be fully automated using RoboSep™. Components: ™ EasySep™ Human CD4+CXCR3- Pre-Enrichment Cocktail, 1.0 mL
™ EasySep™ Magnetic Particles, 2 x 1.0 mL
™ EasySep™ Human CCR6 Positive Selection Cocktail, 0.5 mL
™ EasySep™ Magnetic Nanoparticles, 1.0 mL
Equipment Required: ™ EasySep™ Magnet (Catalog #18000), or
™ “The Big Easy” EasySep™ Magnet (Catalog #18001), or
™ RoboSep™ (Catalog #20000)
