详细信息:The EasySep™ HLA Whole Blood CD3 Positive Selection is designed to isolate CD3+ cells from fresh whole blood by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD3 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD3 antigen is expressed on all T cells and NKT cells. Components: ™ EasySep™ HLA Whole Blood CD3 Positive Selection Cocktail, 3 x 1.0 mL
™ EasySep™ Magnetic Particles, 3 x 1.0 mL
™ EasySep™ HLA FCXM Blocking Solution, 5 x 2.0 mL
™ EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL
Equipment Required: ™ “The Big Easy” EasySep™ Magnet (Catalog #18001), or
™ RoboSep™ (Catalog #20000)