详细信息:The EasySep™ HLA CD3 Positive Selection Kit (Catalog #18051HLA) is designed to isolate CD3+ cells from fresh or previously frozen peripheral blood mononuclear cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD3 and dextran-coated magnetic particles. This cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD3 antigen is expressed on all T cells and CD56+ NKT cells. Positively selected cells are immediately ready for flow cytometric crossmatch analysis (when used with EasySep™ HLA FCXM Blocking Solution, included in kit), serology, or chimerism analysis. Components: ™ EasySep™ HLA CD3 Positive Selection Cocktail, 1.0 mL
™ EasySep™ Magnetic Particles, 1.0 mL
™ EasySep™ HLA FCXM Blocking Solution, 5 x 2.0 mL
Equipment Required: ™ EasySep™ Magnet (Catalog #18000), or
™ “The Big Easy” EasySep™ Magnet (Catalog #18001), or
™ RoboSep™ (Catalog #20000)
