详细信息:The EasySep™ HLA B Cell Positive Selection Kit is designed to isolate CD19+ and CD20+ cells from fresh or previously frozen peripheral blood mononuclear cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD19, CD20 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD19 antigen is present on mature B cells and B cell progenitors, but is lost upon maturation to plasma cells. The antigen CD20 is expressed on all stages of B cell development except on pro B cells or plasma cells. Components: ™ EasySep™ HLA B Cell Positive Selection Cocktail, 1.0 mL
™ EasySep™ Magnetic Nanoparticles, 1.0 mL
Equipment Required: ™ EasySep™ Magnet (Catalog #18000) or
™ “The Big Easy” EasySep™ Magnet (Catalog #18001) or
™ RoboSep™ (Catalog #20000).
