详细信息:The EasySep™ HLA Whole Blood B Cell Positive Selection Kit is designed to isolate B cells from fresh whole blood by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD19, CD20 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD19 antigen is present on mature B cells and B cell progenitors, but is lost upon maturation to plasma cells. The antigen CD20 is expressed on all stages of B cell development except on pro B cells or plasma cells. Components: ™ EasySep™ HLA Whole Blood B Cell Positive Selection Cocktail, 3 x 1.0 mL
™ EasySep™ Whole Blood Magnetic Particles, 3 x 1.0 mL
™ EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL
Equipment Required: ™ “The Big Easy” EasySep™ Magnet (Catalog #18001), or
™ RoboSep™ (Catalog #20000)
