详细信息:The EasySep™ HLA Whole Blood Lymphoid Positive Selection Kit is designed to isolate CD3+ and CD19+ cells from buffy coat or whole blood by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD3, CD19 and dextran-coated magnetic particles. The antibodies are bound in bispecific Tetrameric Antibody Complexes. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD3 antigen is expressed on T cells and NK cells while the CD19 antigen is expressed on B cells. Components: ™ EasySep™ HLA Whole Blood Lymphoid Positive Selection Cocktail, 3 x 1.0 mL
™ EasySep™ Magnetic Particles, 3 x 1.0 mL
™ EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL
Equipment Required: ™ “The Big Easy” EasySep™ Magnet (Catalog #18001), or
™ RoboSep™ (Catalog #20000)
