详细信息:The EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion is designed to isolate monocytes and CD14+CD16+ monocytes from fresh or previously frozen peripheral blood mononuclear cells by negative selection. The CD14+CD16+ subset of monocytes (~10% in blood of healthy individuals) has characteristics of tissue macrophages and expands greatly in acute and chronic inflammatory disease. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD3, CD19, CD20, CD56, CD66b, CD123, glycophorin A and dextran-coated magnetic particles. The cocktail also contains and antibody to human Fc receptor to minimize nonspecific binding. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. For applications in which removal of all CD16+ cells is desired, we recommend the EasySep™ Human Monocyte Enrichment Kit (Catalog #19059), which contains anti-CD16. Components: ™ EasySep™ Human Monocyte Enrichment Cocktail without CD16 Depletion, 1.0 mL
™ EasySep™ Magnetic Particles, 1.0 mL
Equipment Required: ™ EasySep™ Magnet (Catalog #18000), or
™ “The Big Easy” EasySep™ Magnet (Catalog #18001), or
™ "Easy 50" EasySep™ Magnet (Catalog #18002), or
™ RoboSep™ (Catalog #20000)
