详细信息:The StemSep™ Human Na™ve CD4+ T Cell Enrichment Kit is designed to isolate na™ve CD4+ T cells from a peripheral blood mononuclear cell suspension by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD8, CD14, CD16, CD19, CD56, glycophorin A and dextran-coated magnetic particles. CD45RO+ cells are targeted for removal with a biotinylated anti-CD45RO antibody, and a bispecific Tetrameric Antibody Complex that recognizes biotin and dextran. Labeled cells are separated using StemSep™ columns and magnet. Components: ™ StemSep™ Human Na™ve CD4+ T Cell Enrichment Cocktail, 2.0 mL (5 x 2.0 mL)
™ Biotinylated Anti-Human CD45RO, 300 ™L (5 x 300 ™L)
™ StemSep™ Magnetic Colloid, 1.5 mL (4 x 1.5 mL)
Equipment Required: ™ StemSep™ Magnet (Catalog #11030, 11050, 11060, or 11070) or a magnet with the strength of at least 0.5 Tesla
™ StemSep™ Negative Selection Columns
