详细信息:Ex Taq Polymerase is an optimized enzyme for long and accurate PCR. The enzyme is supplied at a concentration of 5 units/μL with a 10X Ex Taq Reaction Buffer and 2.5 mM dNTP Mix.
Unit definition: One unit is the amount of the enzyme that will incorporate 10 nmol of dNTP into acid-insoluble products in 30 minutes at 74°C with activated salmon sperm DNA as the template-primer.
Reaction mixture for unit definition: 25mM TAPS pH9.3 at 25°C; 50mM KCl; 2 mM MgCl2; 1mM 2-mercaptoethanol; 200μM each dATP, dGTP, dTTP; 100μM alpha P32-dCTP; 0.25mg/ml activated salmon sperm DNA
