详细信息:The MultiDrugQuant™ Assay Kit* is used for the functional determination of multidrug resistance in living tumor cells. The kit provides a fast, sensitive and quantitative method for measuring the drug transport activity of the clinically most important multidrug resistance proteins: MDR1 (P-glycoprotein) and MRP1. The assay is based on determining fluorescence intensities using a flow cytometer after a short in vitro incubation of the cell suspension with the dye calcein-acetoxymethyl ester (calcein AM), in the presence or absence of selective inhibitors of MDR1 and MRP1. This testing approach provides a separate measure of multidrug resistance for both MDR1 and MRP1. Since the assay includes an internal standardization, the results of the test, the MDR activity factor (MAF) values provide valuable opportunity for the intra-laboratory and inter-laboratory comparison of multidrug resistance of tumor cells. The MAF values obtained in the MultiDrugQuant™ Assay assist the user to monitor drug sensitivity of malignancy.
