详细信息:A quantitative assay for tumor transendothelial migration has been described using a modified Boyden chamber system, (Okada, 1994; Li, 1999; Laferriere, 2001). The Boyden Chamber system is a two-chamber system with a porous membrane providing an interface between these two chambers. Endothelial cells are cultured on top of the porous membrane that is coated with an extracelluar matrix (ECM) protein. A tumor cell suspension is added above the endothelial monolayer. The invasion of tumor cells across the endothelium is determined by measuring the number of cells that migrate to the lower chamber.
The Millipore QCM™ Tumor Transendothelial Cell Migration Assay– Fluorometric utilizes an 8 ™m pore size cell culture insert, as that is appropriate for most cancer cell lines. The upper side of the cell culture insert is coated with fibronectin to support the attachment and growth of endothelial cells. It allows investigators to compare the invasiveness of different tumor cell lines, and to evaluate the effects of various factors influencing the process. The Millipore QCM™ Tumor Transendothelial Cell Migration Assay– Fluorometric provides pre-coated inserts to reduce the assay time, and a CellTrackerTM Green CMFDA for tumor cell labeling. The CellTrackerTM Green CMFDA can help researcher to differentiate tumor cells from endothelial cells, won’t affect the cell migration function and can be quantitated by a spectrofluorometer.
