详细信息:Millipore’s QCM Leukocyte Transendothelial Cell Migration Assay - Fluorometric provides an efficient model to analyze the ability of leukocytes to migrate through the endothelium. The assay is designed with a 3 ™m pore size cell culture insert, appropriate for most leukocyte migration. The upper side of the cell culture insert is coated with fibronectin to support the optimal attachment and growth of endothelial cells. The assay allows investigators to evaluate the effects of various factors that influence the transendothelial migration process.
Precoated cell culture inserts are provided in the Millipore QCM Leukocyte Transendothelial Cell Migration Assay to significantly reduce assay time. Additionally, the assay allows quantitative analysis of leukocyte migration. Leukocytes were labeled with CellTrackerTM Green CMFDA that can help researchers to differentiate tumor cells from endothelial cells. The CellTrackerTM Green CMFDA will not affect the cell migration function and can be quantified by a spectrofluorometer.
