详细信息:A quantitative assay for tumor transendothelial migration has been described using a modified Boyden chamber system, (Okada, 1994; Li, 1999; Laferriere, 2001). The Boyden Chamber system is a two-chamber system with a porous membrane providing an interface between these two chambers. Endothelial cells are cultured on top of the porous membrane that is coated with an extracelluar matrix (ECM) protein. A tumor cell suspension is added above the endothelial monolayer. The invasion of tumor cells across the endothelium is determined by measuring the number of cells that migrate to the lower chamber.
Millipore's QCM Tumor Cell Transendothelial Cell Migration Assay - Colorimetric provides an efficient model to analyze the ability of tumor cells to invade the endothelium. The assay is designed with an 8 ™m pore size cell culture insert, appropriate for most cancer cell lines. The upper side of the cell culture insert is coated with fibronectin to support the optimal attachment and growth of endothelial cells. The assay allows investigators to compare the invasiveness of a variety of tumor cell lines, and to evaluate the effects of various factors influencing the process.
Precoated cell culture inserts are provided in the Millipore QCM Tumor Cell Transendothelial Cell Migration Assay to significantly reduce assay time. Additionally, the assay allows quantitative analysis of tumor cell migration. Following incubation of tumor cells with the endothelial cell layer, invasive tumor cells are stained and quantified. In a departure from traditional Boyden methodology, stain is eluted with extraction buffer, transferred to a microplate, and measured spectrophotometrically. (Prior to elution, the investigator has the option of counting cells individually, if desired.) Spectrophotometric absorbance correlates with cell migrati
