应用:The CHEMICON™ Endothelial Cell Adhesion Assay Kit is a versatile tool for assessing and quantifying cell to cell interaction of human endothelial cells with leukocytes, or other cell types, under a variety of conditions. The kit is ideal for the assessment of specific cell surface integrins and surface receptors on human endothelial cells, monitoring in vitro cell differentiation, assessing endothelial adhesion blocking, or screening potential cell adhesion
详细信息:The CHEMICON™ Endothelial Cell Adhesion Assay kit (ECM645) allows the researcher to test a variety of cell types that interact with activated or inactivated endothelial cell layers. Human umbilical vein endothelial cells (HUVECs) from ATCC™ (CRL-1730) or Cambrex™ (CC-2519) are ideal for measuring endothelial adhesion activity with the assay. The CHEMICON™ Endothelial Cell Adhesion Assay kit includes a 96 well tissue culture treated fluorescence microtiter plate and reagents that allow for large-scale screening and quantitative comparison of multiple samples/cell line adhesion to an endothelial monolayer. Once endothelial cells are seeded in the tissue culture plate, they can be treated with cytokines, chemokines, transcription regulators, or selective adhesion inhibitors. The protein synthesis inhibitor Cycloheximide and the RNA synthesis inhibitor Actinomycin D are provided as controls for adhesion protein expression studies. Treatment of endothelial cells, such as human umbilical vein endothelial cells (HUVECs), with pro-inflammatory cytokines such as Interleukin-1™™™ eta or Tumor Necrosis Factor-a can induce expression of ECAMs, as well as other adhesion molecules. The kit is supplied with both cytokines.
The viable cell fluorescent compound Calcein-AM™ is provided for labeling test cells, such as leukocytes (granulocytes, monocytes, lymphocytes), fibroblasts, neural stem cells etc., and quantifying their adhesion by comparing bound versus unbound cells. The kit includes three Mouse anti-human monoclonal mAbs for adhesion blocking studies of ICAM-1, VCAM, and E-selectin endothelial cell surface markers. These may be used to verify changes in expression level of adhesion molecules, or effects of integrins upon endothelium activation. This simple and high-throughput kit format allows for the monitoring of the effects of a variety of conditions and compounds on endothelial adhesion. CHEMICON™ also offers a variety of antibodies to leukocyte adhesion molecules and surface antigens.
