详细信息:MEL-2 is a human embryonic stem cell line that has been approved for stem cell derivation by the Australian National Health and Medical Research Council. They are provided at passage 12, which is ideal for maximizing the stable lifespan of the cell line. Later passage cell lines (>20) can have a limited workable shelf-life, since karyotype stability may decrease, and pluripotent characteristics (morphology, marker expression) may shift to reflect a multipotent state. The early passage MEL lines provide extended research time in a stable, pluripotent state. The MEL cell lines grow as well defined colonies, with compact cells displaying high nuclear to cytoplasmic ratios and prominent nucleoli. They have been extensively tested with HEScGRO Animal-Component-Free Medium from Millipore, and have been shown to maintain complete pluripotency. MEL-2 has a stable XX karyotype
