详细信息:Millipore’s FlowCellect™ Multi-STAT Activation Kit is designed to simultaneously detect the phosphorylation of the most commonly studied STAT proteins through cytokine or growth factor mediated pathway activation. The kit will also allow a researcher to quickly profile the status of constitutive activation of STAT1, STAT3, and STAT5 within a population of cells or primary tumors.
The ability to detect the activation of multiple STAT proteins simultaneously allows researchers to determine and profile the STAT signaling pathways within a cell type of interest. Alterations in these signaling profiles can be used to monitor the effect of a particular upstream treatment targeting the STAT pathways.
Antibodies against phospho-STAT1, 3, and 5A/B are provided in the kit to monitor the activation state of each STAT within a given cell type. U937 cells were stimulated with Gamma Interferon, IL-6, and GM-CSF simultaneously, to activate STAT1, STAT3, and STAT5A/B, respectively. When performing a three color stain, triple positive cell populations were detected.
Sufficient reagents are provided for 25 3-color samples. The kit includes all optimized fluorescently labeled antibodies and buffers necessary for cell preparation and analysis. Detailed assay instructions are included to assist in analysis and to ensure the correct cell concentration is obtained during acquisition of sample data.
