详细信息:Millipore’s FlowCellect™ EGFR/STAT3 Activation Detection kit includes two directly conjugated phospho specific antibodies: pEGFR(Tyr1173)-Alexa Fluor™ 488 and pSTAT3 (Tyr705)-Alexa Fluor™ 647, along with optimized fixation, permeabilization, wash, and assay buffers to provide researchers the ability to detect the activation of EGFR and STAT3 at the receptor and downstream signaling level.
Antibodies against phospho-EGFR and phospho-STAT3 are provided in the kit to measure the activation state of each protein. A431 cells were used as a model system to demonstrate EGF mediated activation of EGFR as well as its downstream transcription activator STAT3. The treated cells were compared to untreated cells to verify the detection of both activated proteins by flow cytometry. When performing a dual color stain, the majority of the treated cells are double positive indicating activation of STAT3 through EGFR.
Sufficient reagents are provided for 25 2-color samples. The kit includes all optimized fluorescent labeled antibodies and buffers necessary for cell preparation and analysis.
