详细信息:Caspase 1 Colorimetric Assay Kit, YVAD.Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's ICE/Caspase-1 Colorimetric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on spectophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate YVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405-nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-1 activity.
For research use only; Not for use in diagnostic procedures
