详细信息:Caspase 10 Fluorometric Assay Kit, AEVD.Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's Caspase-10 Fluorometric Protease Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence AEVD. The assay is based on detection of cleavage of substrate AEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). AEVD-AFC emits blue light (lmax=400 nm); upon cleavage of the substrate by caspase-10 or related caspases, free AFC emits a yellow-green fluorescence (lmax=505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in AEVD-dependent caspase activity.
