详细信息:The α-Synuclein ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of α-Synuclein protein in whole cell or tissue extracts (Figure 1). The kit utilizes a 96-well microtiter plate that is pre-coated with an α-Synuclein specific capture antibody. A test sample or the protein standard provided in the kit is then added to each test well and incubated allowing for α-Synuclein antigen to be captured. The plate is subsequently washed to remove any unbound non-specific material and then incubated with a detection antibody that is specific to α-Synuclein. Any unbound detection antibody is washed away prior to a final incubation with HRP conjugated secondary antibody. After the addition of TMB substrate and stop solution, the absorbance is measured at 450 nm using a plate reader. This enzymatic detection format allows for sensitive and specific detection of α-Synuclein protein in test samples.
The entire assay takes less than 5 hours to complete with minimal hands-on time. For ease of use many of the reagents are supplied in a ready-to-use formulation. The kit also includes an α-Synuclein protein standard that is run as a positive control to develop a standard curve.
