详细信息:Millipore’s FlowCellect™ Human Memory B Cell Identification Kit includes three directly conjugated antibodies: Anti-Human CD5-FITC, Anti-Human CD19-APC, and Anti-Human CD27-PE, along with optimized assay buffers to provide researchers the ability to phenotypically distinguish cell types.
Antibodies against CD5 and CD19 are provided in the kit to phenotype cells by their surface marker expression. CD5 is used to identify B-cell subsets, while CD19 is a mature B-cell marker. CD27 is used to phenotypically identify human memory B cells, which can serve useful utility in monitoring the function of this B cell subset during certain disease models. Evaluation of memory B cells is important in determining the capacity of the immune system in producing functional and class-switched antibodies.
All FlowCellect kits are optimized on guava™ bench top flow cytometers. FlowCellect kits can be used on any flow cytometer following the same protocol providing researchers a reliable and fully validated solution to study and identify regulatory B cells right in the comfort of their own lab. All three antibodies provided in the kit are carefully titrated and optimized together to ensure maximal performance when run in multiplex, alleviating the need for any additional optimization.
