详细信息:Millipore’s FlowCellect™ Mouse Breg Identification Kit includes three directly conjugated antibodies: Anti-Mouse CD5-APC, Anti-Mouse CD19-FITC, and Anti-Mouse CD1d-PE, along with optimized assay buffers and a blocking antibody to provide researchers the ability to phenotypically distinguish cell types.
Antibodies against CD5 and CD19 are provided in the kit to phenotype cells by their surface marker expression. CD5 is used to identify B-cell subsets, while CD19 is a mature B-cell marker. CD1d is used to phenotypically identify mouse regulatory B cells, which can serve useful utility in identifying the presence of this B cell subset during certain disease models. Purified Anti-CD16/32 is included for blocking non-specific binding of immunoglobulin to the Fc receptors.
All FlowCellect kits are optimized on guava™ bench top flow cytometers. FlowCellect kits can be used on any flow cytometer following the same protocol providing researchers a reliable and fully validated solution to study and identify regulatory B cells right in the comfort of their own lab. All three antibodies provided in the kit are carefully titrated and optimized together to ensure maximal performance when run in multiplex, alleviating the need for any additional optimization.
