详细信息:Millipore’s FlowCellect™ Bcl-2 Activation Dual Detection kit includes two directly conjugated antibodies: Anti-Bcl-2-Alexa Fluor™ 488 and Anti-pBcl-2 (Serine 70)-PE, along with optimized fixation, permeabilization, wash, and assay buffers necessary for cell preparation and analysis. Millipore’s FlowCellect™ Activation Dual Detection Kit contains sufficient reagents for 25 2-color samples. Detailed assay instructions are included to assist in your analysis and to ensure that the correct cell concentration is obtained during acquisition of sample data.
Millipore’s FlowCellect™ Bcl-2 Activation Dual Detection Kit is designed to enable a researcher a quick and easy way to detect total Bcl-2 expression as well as the phosphorylation of Bcl-2 at serine 70 simultaneously. Millipore’s FlowCellect™ Activation Dual Dectection Kit was developed and tested using Jurkat cells treated with staurosporine, but the kit can be used with other human cell lines to determine the effect of other chemical reagents on Bcl-2 expression and phosphorylation.
