详细信息:Millipore’s FlowCellect™ PI3K Activation Dual Detection kit includes two directly conjugated antibodies, a phospho-specific Anti-phospho-Akt (Ser473)-Alexa Fluor™ 488 and an Anti-Akt-Alexa Fluor™ 647 conjugated antibody to measure total levels of Akt. This two color flow cytometry kit is designed to measure the extent of Akt phosphorylation relative to the total Akt expression in any given cell population. By doing such, the levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of Akt activation after stimulation. Moreover, simultaneous measurement of both total and phospho-Akt confirms target specificity of the phosphorylation event. Together, a total and phospho antibody duo performed in multiplex provides an enhanced and more reliable detection of the phospho:total ratio within a mixed population.
All FlowCellect kits are supplied with optimized fixation, permeabilization, wash, and assay buffers to provide researchers the ability to detect the activation of Akt in flow applications. Sufficient reagents are provided for 25 two-color samples. The kit includes all optimized fluorescently labeled antibodies and buffers necessary for cell preparation and analysis.
