详细信息:Millipore’s FlowCellect Annexin Red Kit provides a rapid and convenient assay to monitor early and late apotosis. The kit includes recombinant Annexin V conjugated to a red sensitive dye, CF647, to provide maximum sensitivity and 7-AAD, a cell impermeant dye which is an indicator of cell membrane structural integrity along with an assay buffer. 7-AAD is excluded from live, healthy cells and early apoptotic cells but stains late stage apoptotic and dead cells with red fluorescence by binding to nucleic acids in the cell. After staining cells with Flowcellect Annexin Red Kit, three populations of cells can be identified in this assay:
™ Non-apoptotic cells: Annexin V(-) and 7-AAD(-)
™ Early apoptotic cells: Annexin V(+) and 7-AAD(-)
™ Late stage apoptotic or dead cells: Annexin V(+) and 7-AAD(+)
The kit permits users to pair the provided reagents with other reagents that fluoresce in the green and yellow channels of the flow cytometer. Sufficient reagents are provided for 100 tests. The kit includes all optimized fluorescently labeled reagents and buffers necessary for cell preparation and analysis.
