详细信息:Millipore’s FlowCellect™ MitoPotential Red Kit is a dual parameter assay kit that includes MitoSense Red , a fluorescent cationic dye that accumulates in the mitochondria and is responsive to mitochondrial potential changes, and the DNA intercalator 7-Aminoactinomycin-D (7-AAD), a membrane impermeant dead cell dye. The simultaneous use of the reagents allows researchers to obtain information on early and late apoptosis in one simple assay. MitoSense Red is excitable by a red laser and fluoresces maximally at 650 nm (Red2 fluorescence on the guava easyCyte 8HT). Uninduced cells with intact mitochondrial membrane potential demonstrate high Red2 fluorescence while cells which have impaired mitochondrial membrane potential depict lower Red2 fluorescence. The 7-AAD dye included in the kit monitors cell membrane permeability changes typically observed later in apoptosis as well as necrotic cell death. 7-AAD is excluded from live, healthy cells as well as early apoptotic cells, which have low red fluorescence. The kit can thus distinguish 3 populations: 1) Live cells with intact mitochondrial membrane 2) Early apoptotic cells with dissipated membrane potential but no 7AAD staining 3) Late Apoptotic Cells or dead cells that have dissipated membrane potential and compromised membranes demonstrate an increase in red fluorescence. The entire assay can be performed in approximately 30 min once cellular samples are ready. Sufficient reagents are provided for 100 tests. The kit includes optimized preformulated dyes and buffers necessary for cell preparation and analysis on a flow cytometer equipped with a blue and red laser.
The FlowCellect™ MitoPotential Red Kit involves simple and fairly rapid assay protocols, requires no compensation and allows for easy interpretation of test results.
