详细信息:Millipore’s FlowCellect™ DNA Damage (Histone H2A.X) and Cell Cycle Analysis Kit is designed to identify the linkage between DNA damage in relation to cell cycle checkpoints in flow cytometry applications. The kit includes a directly conjugated Anti-phospho-Histone H2A.X (Ser139) Alexa Fluor™ 488 antibody plus a DNA dye (Propidium Iodide) which allows the researcher to perform bivariate analysis to identify where DNA damage takes place relative to the positioning within the cell cycle. The kit includes an optimized protocol with all of the necessary components to provide the researcher a true “plug and play” design for their research when studying DNA damage and cell cycle checkpoints. This immunocytochemical method is very sensitive, and it offers an assay to screen anti-tumor drugs at every phase of the cell cycle.In all, a comprehensive understanding of DNA damage and its association with cell cycle behavior can provide the researcher with useful information which will be important in interpreting the intrinsic nature of cell proliferation, apoptosis, DNA repair, and assist in the development of anti-neoplastic agents. Millipore’s FlowCellect™ DNA Damage (Histone H2A.X) and Cell Cycle Analysis Kit is designed to allow the researcher to easily identify, discriminate, and quantitate cells undergoing DNA damage relative to its positioning within the cell cycle.
All FlowCellect kits are optimized on guava™ bench top flow cytometers. FlowCellect kits can be used on any flow cytometer following the same protocol providing researchers a reliable and fully validated solution to study DSBs and cell cycle distribution right in the comfort of their own lab. This kit contains optimized fixation, permeabilization, wash, and flow buffers to provide researchers with a complete solution for bivariate analysis to assess DNA damage in relation to positioning within the cell cycle.
