详细信息:Millipore’s FlowCellect™ Multi-Color DNA Damage Response Kit is designed to enable a researcher a quick and easy way to detect the phosphorylation state of ATM, SMC1 and Histone H2A.X by flow cytometry. Millipore’s FlowCellect™ Multi-Color DNA Damage Response Kit was developed and tested using the DNA damaging reagent, Etoposide, in HeLa cells as a model system but the kit can be used with other human cell lines to determine the effect of mechanical and chemical reagents that can induce DNA damage through the ATM dependent pathway.
Millipore’s FlowCellect™ Multi-Color DNA Damage Response Kit contains sufficient reagents for 25 3-color samples. The kit includes three optimized fluorescently labeled antibodies and buffers necessary for cell preparation and analysis. Detailed assay instructions are included to assist in your analysis and to ensure that the correct cell concentration is obtained during acquisition of sample data.
