详细信息:The Non-Radioactive Chemiluminescent NFκB p50/p65 Transcription Factor Assay kit is provided in a 96-well format. During the assay, the Capture Probe, a double stranded biotinylated oligonucleotide containing the flanked DNA binding consensus sequence for NFκB (5'-GGGACTTTCC-3'), is mixed with cellular (nuclear) extract in the Transcription Factor Assay Buffer provided. When incubated together, the active form of NFκB contained in the nuclear extract查看全部。NFκB p50/p65 EZ-TFA Transcription Factor Assay (Chemiluminescent)